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pcr2.1-topo cloning kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pcr2.1-topo cloning kit
    Pcr2.1 Topo Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr2%2E1/topo+ta+cloning+kit/pm40658726-102-23-26
    Average 90 stars, based on 1 article reviews
    pcr2.1-topo cloning kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Purification:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Polymerase Chain Reaction:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Gel Extraction:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Cloning:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Plasmid Preparation:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    TA Cloning:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Clone Assay:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Methylation:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Sequencing:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Over Expression:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Construct:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Mutagenesis:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Expressing:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.

    Virus:

    Article Title: TFEB activation hallmarks antigenic experience of B lymphocytes and directs germinal center fate decisions.
    Article Snippet: For site-directed mutagenesis, the cDNA was cloned into the plasmid pCR2.1 (Invitrogen).

    Article Title: A transposable element prevents severe hemophilia B and provides insights into the evolution of new- and old world primates.
    Article Snippet: After gel extraction, the DNA was transferred into pCR2.1 (Invitrogen) for sequencing.

    Article Title: Bivalent chromatin instructs lineage specification during hematopoiesis.
    Article Snippet: Article Bivalent chromatin instructs lineage specification during hematopoiesis

    Article Title: The Sole Essential Low Molecular Weight Tropomyosin Isoform of Caenorhabditis elegans Is Essential for Pharyngeal Muscle Function.
    Article Snippet: Tropomyosin is an actinbinding protein that plays roles ranging from regulating muscle contraction to controlling cytokinesis and cell migration.. The simple nematode Caenorhabditis elegans provides a useful model for studying the core functions of tropomyosin in an animal, having a relatively simple anatomy and a single tropomyosin gene, lev11, that produces seven isoforms.. Three higher molecular weight isoforms regulate the contraction of body wall and other muscles, but comparatively less is known of the functions of four lower molecular weight isoforms (LEV11C, E, T, U).

    Article Title: Fluoxetine and Sertraline Potently Neutralize the Replication of Distinct SARS-CoV-2 Variants.
    Article Snippet: To assess M- and N-gene copy numbers, the M- and N-genes were partially cloned into pCR2.1 (Thermo Fisher Scientific) or pMiniT 2.0 (NEB, Ipswich, MA, USA), respectively.

    Article Title: 1 H, 13 C, and 15 N resonance assignments and solution structure of the N-terminal divergent calponin homology (NN-CH) domain of human intraflagellar transport protein 54.
    Article Snippet: 1 RIKEN, Systems and Structural Biology Center, 1-7-22 Suehiro-cho, Tsurumi, Yokohama 230-0045, Japan 2 RIKEN Center for Biosystems Dynamics Research, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 2300045, Japan 3 Faculty of Pharmacy and Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo 202-8585, Japan 4 Institute of Advanced Energy, Graduate School of Energy Science, Kyoto University, Gokasho, Kyoto, Uji 611-0011, Japan 5 RIKEN Yokohama NMR Facility, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 6 Tatsuo Miyazawa Memorial Program, RIKEN Genomic Sciences Center, Yokohama 230-0045, Japan 7 Institute of Biophysical Chemistry, Goethe-University Frankfurt am Main, Max-von-Laue-Str.. 9, Frankfurt am Main 60438, Germany 8 Institute of Molecular Physical Science, ETH Zurich, Vladimir-Prelog-Weg 2, Zurich 8093, Switzerland 9 Department of Chemistry, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji, Tokyo 1920397, Japan 10 RIKEN Structural Biology Laboratory, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan 11 RIKEN Cluster for Science, Technology and Innovation Hub, 1-7-22 Suehiro-cho, Tsurumi, Yokohama, Yokohama 230-0045, Japan Abstract The intraflagellar transport (IFT) machinery plays a crucial role in the bidirectional trafficking of components necessary for ciliary signaling, such as the Hedgehog, Wnt/PCR, and cAMP/PKA systems.. Defects in some components of the IFT machinery cause dysfunction, leading to a wide range of human diseases and developmental disorders termed ciliopathies, such as nephronophthisis.

    Article Title: Metabolic Engineering and Process Intensification for Muconic Acid Production Using Saccharomyces cerevisiae
    Article Snippet: pCR2.1 , Base plasmid for overexpression constructs , Thermo Fisher Scientific.

    Article Title: The NIPBL-gene mutation of a Cornelia de Lange Syndrome patient causes deficits in the hepatocyte differentiation of induced Pluripotent Stem Cells via altered chromatin-accessibility
    Article Snippet: The PCR-amplified DNA fragments were also sub-cloned in pCR2.1 (Thermo Fisher Scientific), and the inserts of 10 independent clones/experimental-group were sequenced to confirm the methylation state of the DPP6 promoter.



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    Thermo Fisher pcr2.1-topo ta
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    https://www.bioz.com/product/pcr2%2E1/topo+ta+cloning+kit/pmc12141343-38-9-14
    Average 90 stars, based on 1 article reviews
    pcr2.1-topo ta - by Bioz Stars, 2026-10
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